http://lod.bco-dmo.org/id/dataset/738228
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-06-07
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Traits of five Symbiodinium genotypes measured at three different nitrogen levels
2018-05-23
publication
2018-05-23
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-12-04
publication
https://doi.org/10.1575/1912/bco-dmo.738228.1
Casey terHorst
California State University Northridge
principalInvestigator
Mary Alice Coffroth
State University of New York at Buffalo
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
documentDigital
Cite this dataset as: terHorst, C., Coffroth, M. (2018) Traits of five Symbiodinium genotypes measured at three different nitrogen levels. Biological and Chemical Oceanography Data Management Office (BCO-DMO). Dataset version 2018-05-23 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.738228.1 [access date]
Symbiodinium genotype responses to nitrogen (Expt 2) Dataset Description: <p>Replicates of each Symbiodinium genotype were grown in low (25mg/L), medium (75mg/L), or high (150mg/L) nitrogen environments, after which were measured physiological parameters, including the number of cells, quantum yield, variable fluorescence, and chlorophyll content.</p> Acquisition Description: <p>Cultures initially isolated from&nbsp;Antillogorgia&nbsp;bipinnata&nbsp;coral colonies collected at Looe Key ( N 24° 32.973' W 81° 22.849; cultures 08-0689.4, 08-0689.6, 08-0691.1) in 2008 and the middle keys new Tennessee Reef (N 24° 45.150'&nbsp; W 81° 45.275'; cultures 13-121, 13-126) in 2013. Culture isolations were performed in the Coffroth lab, University at Buffalo. Physiological measurements were measured in the terHorst lab, California State University, Northridge. Quantum yield and fluorescence&nbsp;yield&nbsp;were measured in an AquaPen-C (Photon Systems Instruments). Chlorophyll concentration was quantified in a Triology Fluorometer. Culture growth rates were quantified by visual counts under a microscope at the beginning and end of the experiment. See Bayliss et al (2019) for further details and results.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1559105 Award URL: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1559105
completed
Casey terHorst
California State University Northridge
818-677-3352
Department of Biology 18111 Nordhoff Street
Northridge
CA
91330-8303
USA
casey.terhorst@csun.edu
pointOfContact
Mary Alice Coffroth
State University of New York at Buffalo
716-645-4871
Department of Geology 126 Cooke Hall
Buffalo
NY
14260
Coffroth@buffalo.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Genotype
Ntreatment
Growthrate
QY
Vfl
Chla
Chlapercell
lncells
Trilogy Laboratory Fluorometer
AquaPen-C (Photon Systems Instruments)
microscope
theme
None, User defined
sample identification
treatment
growth
Fv2Fm
chlorophyll a
cell_concentration
featureType
BCO-DMO Standard Parameters
Fluorometer
Fluorometer
Microscope-Fluorescence
instrument
BCO-DMO Standard Instruments
Coffroth_2016
service
Deployment Activity
SUNY - Buffalo lab
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
RUI: Collaborative Research: Genetic variation as a driver of host and symbiont response to increased temperature on coral reefs
https://www.bco-dmo.org/project/632538
RUI: Collaborative Research: Genetic variation as a driver of host and symbiont response to increased temperature on coral reefs
<p><em>Description from NSF award abstract:</em><br />
On coral reefs, mutualisms with single celled algae (Symbiodinium) and reef species literally and figuratively form the foundation of reef ecosystems. Coral reefs are among the most threatened ecosystems under a changing climate and are rapidly declining due to increasing levels of environmental stress, namely increased temperatures. Climate change is resulting in even warmer ocean temperatures that threaten associations between Symbiodinium and their hosts. In this project the investigators examine the genetic diversity of Symbiodinium and the potential for this important species to evolve in response to temperature. The project will also address whether the ecological and evolutionary dynamics of the Symbiodinium population affect the performance of their host. If so, this suggests that the evolution of microscopic organisms with short generation times could confer adaptation to longer-lived host species on ecologically and economically vital coral reefs. Given that diversity is already being lost on many reefs, considering how evolutionary changes in Symbiodinium will affect reef species is crucial for predicting the responses of reefs to future climate change. This project provides training for two graduate students and several undergraduates at a Hispanic-serving institution. This work includes outreach to the students and the general public through the Aquarium of Niagara, local K-12 schools, and web-based education modules.</p>
<p>The effects of evolution on contemporary ecological processes are at the forefront of research in evolutionary ecology. This project will answer the call for experiments elucidating the effects of genetic variation in Symbiodinium performance and the effect on the response of the holobiont (host and symbiont) to increased temperature. These experiments examine the effects of temperature through both ecological and evolutionary mechanisms and will determine the relative importance of adaptation and acclimatization in replicated experimental populations. The investigators will examine how genetic variation within a species (Symbiodinium antillogorgium) affects symbiont performance in culture and in the host and how this affects the response of the holobiont to increased temperature. Further, the project examines whether holobiont response to increased temperature associated with climate change depends on particular GxG host-symbiont combinations. Moreover, the investigators will examine the effects of symbiont history on mutualist hosts, which have been largely ignored in eco-evolutionary studies. These experiments provide a first step in predicting whether invertebrate hosts on coral reefs will respond to global change via adaptation of their symbionts.</p>
Host Symbiont Temp Response
largerWorkCitation
project
eng; USA
biota
oceans
SUNY - Buffalo lab
-81.75458
-80.26195
24.54955
25.1326
2016-01-03
2016-02-28
Florida Keys, Caribbean
0
BCO-DMO catalogue of parameters from Traits of five Symbiodinium genotypes measured at three different nitrogen levels
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/738236.rdf
Name: Genotype
Units: unitless
Description: Genetic idenetifier
http://lod.bco-dmo.org/id/dataset-parameter/738237.rdf
Name: Ntreatment
Units: unitless
Description: Nitrogen level
http://lod.bco-dmo.org/id/dataset-parameter/738238.rdf
Name: Growthrate
Units: cells/day
Description: Population growth rate
http://lod.bco-dmo.org/id/dataset-parameter/738239.rdf
Name: QY
Units: unitless ratio
Description: Quantum Yield
http://lod.bco-dmo.org/id/dataset-parameter/738240.rdf
Name: Vfl
Units: unitless
Description: Variable Fluorescence
http://lod.bco-dmo.org/id/dataset-parameter/738241.rdf
Name: Chla
Units: Relative Fluorescence Units
Description: Total Chlorophyll per sample
http://lod.bco-dmo.org/id/dataset-parameter/738242.rdf
Name: Chlapercell
Units: Relative Fluorescence Units
Description: Chlorophll per cell
http://lod.bco-dmo.org/id/dataset-parameter/738243.rdf
Name: lncells
Units: Ln(#cells)
Description: Log of Cell Count
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://www.bco-dmo.org/dataset/738228/data/download
download
onLine
dataset
<p>Cultures initially isolated from&nbsp;Antillogorgia&nbsp;bipinnata&nbsp;coral colonies collected at Looe Key ( N 24° 32.973' W 81° 22.849; cultures 08-0689.4, 08-0689.6, 08-0691.1) in 2008 and the middle keys new Tennessee Reef (N 24° 45.150'&nbsp; W 81° 45.275'; cultures 13-121, 13-126) in 2013. Culture isolations were performed in the Coffroth lab, University at Buffalo. Physiological measurements were measured in the terHorst lab, California State University, Northridge. Quantum yield and fluorescence&nbsp;yield&nbsp;were measured in an AquaPen-C (Photon Systems Instruments). Chlorophyll concentration was quantified in a Triology Fluorometer. Culture growth rates were quantified by visual counts under a microscope at the beginning and end of the experiment. See Bayliss et al (2019) for further details and results.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Trilogy Laboratory Fluorometer
Trilogy Laboratory Fluorometer
PI Supplied Instrument Name: Trilogy Laboratory Fluorometer PI Supplied Instrument Description:Used to measure chlorophyll concentrations. Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
AquaPen-C (Photon Systems Instruments)
AquaPen-C (Photon Systems Instruments)
PI Supplied Instrument Name: AquaPen-C (Photon Systems Instruments) PI Supplied Instrument Description:Used to measure quantum yield and fluorescence yield. Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
microscope
microscope
PI Supplied Instrument Name: microscope PI Supplied Instrument Description:Used to count cells for growth rate calculations. Instrument Name: Microscope-Fluorescence Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB06/
Deployment: Coffroth_2016
Coffroth_2016
SUNY-Buffalo
laboratory
Coffroth_2016
Mary Alice Coffroth
State University of New York at Buffalo
SUNY-Buffalo
laboratory