+--------------------------------------------------------------------------+ | ATLANTIS CRUISE SYNOPSIS | +--------------------------------------------------------------------------+ Voyage - Leg: AT 15-07 Voyage Dates: 10-29 July 2006 Chief Sci(s): Lisa Levin (Levin) Address: Scripps Institution of Oceanography 9500 Gilman Drive La Jolla, CA 92093-0218 Phone: 858-534-3579 Email: llevin @ucsd.edu Cruise Objective: Objectives: Study the ecology, physiology and evolution of methane seep sediment faunas (with focus on dorvilleid polychaetes) in association with microbial and geochemical environments. Science Activities: Specific tasks will include (1) deployment and recovery of colonization tray experiments (12 at Eel R., 12 at Hydrate ridge) to test settlement response to sulfides (use of gear elevator+sub). (2) collection of sediment in push cores/box cores for measurements of sulfide, sulfate reduction (35S), animal distributions/nutritional properties, shipboard incubation experiments with 13C tracers, nitrogen fixation, microbial consortia, sediment properties etc. (3) deployment and recovery of carbonate colonization substrates (4) large animal collections via biobox, scoop and slurp for nutritional studies (fatty acid, lipids, isotopes). (5) photographic surveys of seep and carbonate epifauna/habitat structure (6) rock collections (carbonates) Night operations will include extensive CTD casts and water sampling + multicoring transects across the oxygen minimum zones off CA/OR (400-1200 m). Operations Area: (updated May 16 EC) N. California (Eel R): two general work areas (all 500-525 m) Southern: 40o 47.19 N 124o 35.70 W Northern: 40o 48.70 N 124o 36.67 W Oregon Hydrate Ridge there are 3 regions we want to dive. North: 44o 40.0184 N 125o 6.0022 W, 599 m South: 44o 34.22 N 125o 8.80 East: 44o 34.31 N 124o 59.89 SSSG Tech: sssg @atlantis.whoi.edu Departure Port: San Diego, CA Agent: Master R/V Atlantis Attn: Scientist's Name c/o Paxton, Shreve & Hays Inc. 2191 Main St. San Diego, CA 92113 Contact: Tom Jenkins tel. (619) 232-8941 fax: (619) 232-3006 telex: 6731029 SHREVE SDG email: marineops@pshinc.net Arrival Port: San Francisco, CA Agent: Master R/V Atlantis Attn: Scientist's Name c/o Vasile Tudoran Transport 819 Ohio Ave. Long Beach, CA 90804 Contact: Vasile Tudoran tel. (562) 882-5590 fax: (562) 434-9800 email: vtudoran@aol.com Use above address for small packages (up to 70 lbs). All items must be delievered five days prior to ship arrival in San Francisco. For items larger than 70 lbs, send directly to our subagent in San Francisco: Master R/V Atlantis Attn: Scientist's Name c/o Western Overseas Corp. 100 Produce Ave., Suite K South San Francisco, CA 94080 Contact: Elvin Briones tel. (650) 952-2955, (650) 989-7387 fax: (650) 952-2978 email: elvinb@westernoverseas.com Installed Scientific 12 & 3.5 kHz Transducers Equipment: ADCP Deionized Water System CTD/Rosette - 24X10L - tubidity, O2, Fluorometer, Transmissometer, Temperature, Conductivity, Pressure (JD 5/4) PAR Underwater PAR Surface Seabeam 12khz Pinger for wire use Fume Hood Refrigerator -70 Deg Freezer Walk-In Cooler Walk-In Freezer (JD 5/4) Anti-Vibration Table / Dark Room for scope work Compressed Air Uncontaminated sea water WHOI-Provided Science Box Corer- (req'd as backup) Tools: Multicorer Isotope Van ALVIN Small capacity Slurp Pump (might need 2) Bio Collection Box (12x12x24 to store scoop bags) Push Cores - 24 cores per dive (std 2.5" ID) Scoop Nets (canvas bags required) Low Temperature probe CTD External digital survey camera (forward looking) Search Sonar Niskin rack - 1L Doppler/GPS Navigation Transponder Navigation - 2 nets; 2 transponders/net In sub camera Gear Elevator (once/day- deploy/recover sample trays) Program-Provided O2 Titration Science Tools: Plankton Net (SIO) - Lisa is checking on Otter Trawl (SIO) (EC 6/15) Box cores for Alvin use Sample trays for deployment Dukane pingers for equipment location If film crew participates, HiDef camera system (WHOI's or other) Shipboard Equipment/Nav: Other Requirements: Night Work Anticipated (CTD and Multi-core) Science Stowage: Scinece gear will be stored int eh hold 2 Pallet size boxes will be stored on beck between cruises Notes: Will need a multicorer (Coring tech will be provided by WHOI). Will need high quality still photo imagery with laser scaling for photos. Notes from EC. Will be using Dukane pingers (37kHz) on deployments Nitrogen tanks (10) - request to leave them on board between cruises Need 1 elevator per day (JD-2/21) ALVIN Push Cores - We will want the maximum - probably 24. We won't be keeping cores but it may take more than 2 days to process them and turn them around Hazarous Material to be used aboard - disposal details to follow Sampling Plan - for Aluwihare Lab participants Goal - to collect bacterial cells, and ancillary data (including nutrients, methane samples for concentration and isotope measurements [Dave can you do this on your mass spec? low end concentrations?], DI13/14C, and DO14/13C samples, bacterial counts, crude species composition (FISH or related technique), DOC, DON, GF/F POC samples [using hand pump]) from 3 types of sites to investigate carbon sources fueling various microbial communities in the water column and to record changes in microbial community structure associated with water column methane gradients. Depth - surface stations wherever the boat happens to be that day. Surface sampling is straightforward. We drop a teflon hose (3-5 m) usually on the starboard side forward of the CTD, pump water up using diaphragm pump powered by compressed air and through in line 0.5 mm and 0.1 mm filters. Integrate all surface samples and store filters in fridge in-between sampling. Stations close to the methane venting sites - deeper in the water column near the site of venting. First, drop the CTD to get T, chl a, O2 going down. Trip the Rosette at several depths on the way up to draw samples for ancillary measurement. CTD drops at about 50 m/minute, which means about 1 hr and 30 min. to descend to 1500 m and sample full profile on the way back (assuming twenty four 10 L bottles on the rosette). It will take us about 40 minutes to sample the profile for various parameters. So let's plan on 2 hours or so for the first CTD and sampling. After that, we would like to send the CTD down again. The depth will be determined based on the vent location. Ideally, we would like to get as close as possible without actually sampling the bottom (!) and huge amounts of vent fluid. When the CTD gets to the desired depth we will trip all bottles at that depth. This sampling scheme usually takes about 1 hour. Once the CTD is onboard we will filter onto 0.5 and 0.1 mm filters directly from the Niskin bottles (again using the compressed air pump and Teflon tubing adapted to fit over the nipples on the Niskin bottles). Based on previous experience this takes about 30 minutes. So, 1 hour for bacterial cell collection. We would like to do as many of these casts as possible on any given night (probably no more than 3 in a practical sense but as many as we can). If we are close to the same site each night we could potentially avoid having to do an ancillary data cast every night. Upper water column sampling - if we can get 3-4 night's worth of the above sampling scheme then we would also like to sample further up in the water column at the interface where water column methane goes from some methane to none at all. The goal is to compare the microbial communities at these sites to the ones that are living close to the methane source. Sampling scheme is as above but likely much faster. We are not particularly picky about whether we are sitting above the same vent or not as long as measurable methane is present in the water column. We will likely integrate samples from a variety of locations in order to increase our sample size. Filters will be stored in the refrigerator after each sampling but ultimately frozen. Filters will need to be shipped back on dry ice. # Casts per night - as many as possible given the restech's schedule (we are not restricted to night sampling from our end). Practically we can maybe do 3 or 4 a night depending on the depth (again this is not limited by our equipment) # Of Days - Whenever we are not steaming (i.e. as many days as possible) Depth - maximum depth will probably be under 2000 m. Shipboard needs - Compressed air (near the CTD, we are bringing one hose), -20 or -80 freezer space and refrigerator space, some dry lab space, Milli-Q water, lots of CTD time Please notify us if radioisotopes are being used or were used by the prior science party. We need a radio-isotope free space since we are making natural abundance measurements (this is particularly important in terms of our sample storage needs). We will bring a small quantity of mercuric chloride, a small quantity (20-40 mL of HCl), and a small quantity of formaldehyde (MSDS sheets will accompany the science party). Otherwise, we just need water. Navy Clearance Status: Granted 07/05/06: WITHIN 100 NM RADIUS OF 40-48N 124-36W DURING PERIOD 130700Z THRU 290700Z JUL 06 WITHIN 100 NM RADIUS OF 44-00N 125-00W DURING PERIOD 130700Z THRU 290700Z JUL 06 Last Modified: 07/24/2006 Check List: Required? Comments US Customs Form No Explosives Clearance No Isotope Use Approval Yes authorization request not received Diplomatic Clearance No SCUBA Diving No