+--------------------------------------------------------------------------+ | ATLANTIS CRUISE SYNOPSIS | +--------------------------------------------------------------------------+ Voyage - Leg: AT 11-28 Voyage Dates: 7 - 16 Jun 2005 Chief Sci(s): Kevin Brown (Brown/Hilton) Address: Scripps Inst. Oceanography UCSD La Jolla, CA 92093-0244 Phone: 858-534-5368 Email: kmbrown@ucsd.edu Cruise Objective: Science Activities: Instrument deployment at cold seeps + chemistry on proximal push cores Operations Area: Costa Rica 8-55.45N; 84-18.45W 1-3km SSSG Tech: sssg@atlantis.whoi.edu Departure Port: Puntarenas, Costa Rica Agent: Master R/V Atlantis Attn: Scientist's Name c/o Vasile Tudoran Transport 819 Ohio Ave. Long Beach, CA 90804 Contact: Vasile Tudoran tel. (562) 882-5590 fax: (562) 434-9800 email: vtudoran@aol.com Local address for large shipments: SERMAR SA Aptdo 26 Puntarenas, Costa Rica Attn: Carlos Aguilar Tel. 011-506-661-1529 Fax 011-506-661-2779 Arrival Port: Puntarenas, Costa Rica Agent: Same as above Installed Scientific CTD - 24X10L - temp, salinity, transmissivity + MET Equipment: Sensor Pinger for wire use (12khz) Refrigerator Walk-in cooler Walk-in freezer Seanet Compressed Air near the CTD Milli-Q water (5-10L/day) WHOI-Provided Science Isotope van Tools: ALVIN Major water samplers -We would like the option to deploy the major sampler if we get on a high rate seep Small capacity slurp pump (2-3 Pelagic pumps w/sample chambers to slurp mats) No known science wiring interface requirements to Alvin Bio collection box - 12"12"X12" Push cores (12 needed) Low temperature probe Heat flow probe CTD (Science party will maintain CTD & download data) DVLNAV, particularly in non LBL areas (Science party will provide JPEG topo maps for underlay) Relay transponder for wire guided "CAT" instrument deployments (transponder on "Mass Surfer" can be used as a relay for that deployment) Niskin Bottles - 5 each 1 liter bottles Program-Provided Mass Surfer Instrument - We are aiming for a Science Tools: negative weight in water of no more than 50 pounds. It indeed does weigh about 950 pounds in air, including the removable weights, which will allow it to float to the surface (the package has a Novatech VHF radio beacon and Xe strobe for location on the surface). The idea is to lower it to the seafloor on a wire with a release. The package has a Datasonics transponder that receives at 11 kHz and transmits at 9 kHz. Alvin will be able to grab it on the side struts and pull it toward the front of the basket. The sub can then move it into position on the seeps after it locates the package on the bottom. Instrument recovery will occur 6-12 months after deployment using a ROV or Alvin. All pressure tests are complete and docs will be provided to Expedition Leader. "CAT" instruments for deployment/moving by Alvin and/or wire drop placement. All pressure tests are complete and docs will be provided to Expedition Leader. Methane sensor from Capsum for the ship's CTD rosette - collect a water column methane profile Shipboard Transponder navigation (possibly 2 nets, dependnig Equipment/Nav: on terrain) Other Requirements: Special equipment handling - lowering instruments to seabed with CTD winch and deploying with acoustic releases provided by science party (Edgetech?). Night work anticipated A CTD cast upon arrival at station on the 7th is requested. Notes: 1 container being shipped from San Diego - Only contents to be loaded 1 container being shipped from Germany - Only contents to be loaded. Contents of containers will be loaded at Puerto Caldera when dock is available, possibly June 3rd. German participants will remove all hazardous material at end of cruise (PI: Werner Brueckmann) Isotopes being shipped to Science Colleague in Costa Rica Sampling Plan - for Aluwihare Lab participants are Jordan T. Watson and Roberta Hansman). Goal - to collect bacterial cells, and ancillary data (including nutrients, methane samples for concentration and isotope measurements [Dave can you do this on your mass spec? low end concentrations?], DI13/14C, and DO14/13C samples, bacterial counts, crude species composition (FISH or related technique), DOC, DON, GF/F POC samples [using hand pump]) from 3 types of sites to investigate carbon sources fueling various microbial communities in the water column and to record changes in microbial community structure associated with water column methane gradients. Depth - surface stations wherever the boat happens to be that day. Surface sampling is straightforward. We drop a teflon hose (3-5 m) usually on the starboard side forward of the CTD, pump water up using diaphragm pump powered by compressed air and through in line 0.5 mm and 0.1 mm filters. Integrate all surface samples and store filters in fridge in-between sampling. Stations close to the methane venting sites - deeper in the water column (MET sensor will be needed to get a general idea of methane concentration and the sensor cannot go below 2000 m) near the site of venting. First, drop the CTD to get T, chl a, O2 and MET going down. Trip the Rosette at several depths on the way up to draw samples for ancillary measurement. CTD drops at about 50 m/minute, which means about 1 hr and 30 min. to descend to 1500 m and sample full profile on the way back (assuming twenty four 10 L bottles on the rosette). It will take us about 40 minutes to sample the profile for various parameters. So let's plan on 2 hours or so for the first CTD and sampling. After that, we would like to send the CTD down again. The depth will be determined based on the MET sensor (if the MET sensor is not working we will have to sample based on vent location). Ideally, we would like to get as close as possible without actually sampling the bottom (!) and huge amounts of vent fluid. When the CTD gets to the desired depth we will trip all bottles at that depth. This sampling scheme usually takes about 1 hour. Once the CTD is onboard we will filter onto 0.5 and 0.1 mm filters directly from the Niskin bottles (again using the compressed air pump and Teflon tubing adapted to fit over the nipples on the Niskin bottles). Based on previous experience this takes about 30 minutes. So, 1 hour for bacterial cell collection. We would like to do as many of these casts as possible on any given night (probably no more than 3 in a practical sense but as many as we can). If we are close to the same site each night we could potentially avoid having to do an ancillary data cast every night. Upper water column sampling - if we can get 3-4 night's worth of the above sampling scheme then we would also like to sample further up in the water column at the interface where water column methane goes from some methane to none at all. The goal is to compare the microbial communities at these sites to the ones that are living close to the methane source. Sampling scheme is as above but likely much faster. We are not particularly picky about whether we are sitting above the same vent or not as long as measurable methane is present in the water column. We will likely integrate samples from a variety of locations in order to increase our sample size. Filters will be stored in the refrigerator after each sampling but ultimately frozen. Filters will need to be shipped back on dry ice. # Casts per night - as many as possible given the restech's schedule (we are not restricted to night sampling from our end). Practically we can maybe do 3 or 4 a night depending on the depth (again this is not limited by our equipment) # Of Days - Whenever we are not steaming (i.e. as many days as possible) Depth - maximum depth will probably be under 2000 m. Shipboard needs - Compressed air (near the CTD, we are bringing one hose), -20 or -80 freezer space and refrigerator space, some dry lab space, Milli-Q water, lots of CTD time and MET sensor on CTD package (Germans are bringing the sensor, we are bring the cable and mount kit). Please notify us if radioisotopes are being used or were used by the prior science party. We need a radio-isotope free space since we are making natural abundance measurements (this is particularly important in terms of our sample storage needs). We will bring a small quantity of mercuric chloride, a small quantity (20-40 mL of HCl), and a small quantity of formaldehyde (MSDS sheets will accompany the science party). Otherwise, we just need water. Navy Clearance Status: Granted 06/01/05: WITHIN 50 NM RADIUS OF 09-01N, 084-34W DURING PERIOD 071600Z THRU 160000Z JUN 05 Last Modified: 01/19/2006 Check List: Required? Comments US Customs Form Yes Explosives Clearance No Isotope Use Approval Yes Diplomatic Clearance Yes Costa Rica SCUBA Diving No