CHLOROPHYLL DATA

Overview

We employ two methods for extracting chlorophyll a, DMS method and Acetone method. Acetone method was applied for only three data set of reference number of OS199703, OS199902, and OS199903. The other data sets were analyzed by DMS method.

DMS Method
Seawater samples collected by CTD+Rosset system were filtered onto 25mm Whatman GF/F filters using gentle suction of 150mmHg. After filtration, the filters were dipped in 6ml of N,N-dimethylformamide (DMF) for extracting chlorophyll a (Chl a). Extractions of chl-a were done in a dark place at -80deg;C. Chl a concentrations were measured with Turner Designs Model 10-AU Field Fluorometer (Parsons et al. , 1984).

Acetone Method
Seawater samples for chlorophyll a (Chl a) were filtered through Whatman GF/F filters (Ø47mm, 0.7µm nominal porosity) at -100 mg Hg vacuum pressure. Filters were folded in aluminum foil and stored at -20°C in a desiccator until analysis. Volumes of samples varied from 200 to 500 ml depending on ambient Chl a concentrations. The filters were placed in test tubes containing 5 ml of 90% aqueous acetone. Extraction took place in the dark at -20°C for 24 h. Chl a concentrations were measured ashore by a Turner Designs Model 10-AU Field Fluorometer following the methods of Parsons et al. (1984).

Data format

ODV (Ocean Data View) format