Each BATS cruise is four to five days duration and occur at biweekly to monthly intervals.The core set of measurements are collected on two hydrocasts, one measurement of integrated primary production and a sediment trap deployment of three days duration.
These cruises usually follow a regular schedule for the sequence and timing of events. Weather, equipment problems and other activities occasionally cause this schedule to be interrupted or rearranged. In the data report for each cruise, the exact schedule actually used should be reported, including the timing and nature of other activities. The schedule described below represents a summary of all the core activities on each cruise in the order that they would be performed barring any other factors.
Immediately after arrival near the station
(31° 50' N, 64° 10' W), the sediment traps are deployed. This trap
array has Multi-traps at 150, 200, and 300 m depths. The trap is free-floating
and equipped with a strobe, radio beacon and an ARGOS satellite transmitter.
The ship remains near the trap for the rest of the sampling period (see
production section
below) resulting in a quasi-Lagrangian
sampling plan. The locations of each cast are reported with the data reports.
The decision to keep the ship near the drifting trap is done for logistical
reasons only. In other studies, casts at a fixed location may be preferred.
2.0 Hydrocasts
The core measurements require 2 hydrocasts
using the 24 place rosette system. The deeper of the two casts is usually
done first. 24 discrete water samples are taken on each cast with the 12
l Niskin bottles. The cast order is as follows:
Cast 1: 0–4200 m. Bottle samples
(24) are collected at 4200, 4000, 3800,3400, 3000 (duplicates), 2600, then
at 200 m intervals until 1400 m, and at 100 m intervals from 300-1400 m.
Cast 2: 0–250 m. 2 bottles are
closed at each of 12 depths of 250, 200, 160,140, 120, 100, 80, 60, 40,
20 and the surface. The extra pair ofbottles are closed at the subsurface
chlorophyll a maximum as determined by the fluorescence profile on the
downcast. Gases,nutrients and dissolved organic matter samples are taken
from this cast, as well as water
samples for particulate organic carbon and particulate nitrogen, pigments
and bacterial abundance.
3.0 Water Sampling
3.1 Sampling begins immediately after
the rosette is brought on board and secured. Care should be taken to protect
the rosette sampling operation from rain, wind, smoke or other variables
which may effect the samples. Oxygen samples are drawn first (if freon
and/or helium is sampled, they should be drawn before the oxygen samples).Two
115 ml BOD bottles are filled from each Niskin and the order of the two
samples is recorded. One set of BOD bottles is for the first oxygen sample,
termed O2 -1 and a different and distinct set is for the second
oxygen sample which is termed the replicate
oxygen sample or O2 - 2 in all data records. After the oxygens,
samples for total CO2 and alkalinity (only taken on cast 2)
are drawn, followed by a single salinity sample. This sampling order is
common to all the bottles in the two casts. The remainder
of the sampling differs depending on the depth.
3.2 The next step in the sampling is drawing particulate organic carbon and nitrogen samples, followed by nutrient samples. Samples for bacterial enumeration are drawn at 3000 and 4000 m and most of the shallow depths. The replicate depths in cast 2 are used for chlorophyll determination, bacterial enumeration and samples for HPLC determination of pigments.
3.3 Deckboard water-processing activities are usually divided into specific tasks. Two or three people draw the water, while one person adds reagents to the oxygen samples and keeps track of the sampling operation. Bottle numbers for each sample at each depth are determined before the cast. All of the sampling people are informed of the sampling scheme and the oversight person ensures that it is being carried out accurately.
4.0 Primary Production
The primary production cast is generally
performed on the second day, depending on the weather, time of arrival
at station, etc. The dawn to dusk in situ production measurement involves
the pre-dawn collection of water samples at 8 depths using trace-metal
clean sampling techniques. A length of Kevlar hydrowire has been mounted
on one of the winches. The bottles are 12 liter Go-Flos with Viton O-rings.
These Go-Flos are acid cleaned with 10% HCl between cruises. The bottles
are mounted on the Kevlar line and depths are measured with a metered block,
or premeasured before the cast, and marked with
tape. These samples are brought back on deck, transferred in the dark to
250 ml incubation flasks, 14 C added and the flasks attached
to a length of polypropylene line at each depth of collection.This array
is deployed with surface flotation which includes a radio beacon and a
flasher. The ship follows this production array during the 12–15 hour period
that it is deployed, occasionally shuttling back to the sediment trap location.
This array is recovered at sunset and processed immediately.
5.0 Sediment Trap Deployment and Recovery
Upon arrival at the BATS station, the
sediment trap array is deployed and allowed to drift free for a 72 hour
period. The array’s location is monitored via the ARGOS transponder and
by regular relocation by the ship. Twice daily, the trap position is radioed
to the ship by BBSR personnel. The rate of drift can be considerable, as
much as 100 km in three days.
6.0 Shipboard Sample Processing
Most of the actual sample analysis for
the short BATS cruises is done ashore at the Bermuda Biological Station
for Research. Oxygen samples are analyzed at sea because of concerns regarding
the storage of these samples for periods of two to three days. Oxygen samples
collected on the last day are sometimes returned to shore for analysis.
All of the other measurements have preservation techniques that enable
the analysis to be postponed. See the individual chapters for details.
For longer cruises, it is strongly recommended that analytical work be
carried out at sea for best results.