PI: Karen Wishner, Marcia Gowing dataset: Zooplankton carbon by size fractions at 0-200m using bongo net tows project: Arabian Sea cruise: TTN-045, JGOFS/Arabian Sea Process cruise 2, inter-monsoon ship: R/V Thomas Thompson Sampling Protocols: Mesozooplankton Biomass Documentation: JGOFS Arabian Sea PIs: Karen Wishner (URI), Marcia Gowing (UCSC) Data Base Organizer and Chief Technician: Celia Gelfman Assistants: Chelsea Donovan, Heidi Franklin, Maureen Koneval, Lauren Lankau, John Maccario, Jennifer Saltzman, Dorothee Schreiber, Cynthia Venn, Kathleen Vignes. Samples were collected with a double 1 m2 MOCNESS (two 1 m2 MOCNESS systems side-by-side), a multiple opening-closing net system with environmental sensors and control of the nets from shipboard (Wiebe et al. 1976, 1985). The nets were 153 micron mesh with a 1 m2 mouth opening (when towed at a 45 degree angle). The net was towed usually at 1.5 - 2.5 kts behind the ship. Wire was payed out at 10 - 25 m/min and hauled in at 5 - 20 m/min. Environmental data from the MOCNESS included time, depth, temperature (Sea-Bird SBE 3), salinity (Sea-Bird SBE 4), light transmission (SeaTech 25 cm beam transmissometer), and oxygen (Sea-Bird SBE 13). The volume filtered through each net was determined by the MOCNESS program, taking into account flow past the system (measured with a modified TSK flowmeter mounted on the net frame) and the towing angle. In some cases, the volume filtered per time from trouble-free portions of a tow was extrapolated to time intervals with electronic problems. Usually 16 discrete samples were collected in an oblique haul from about 1000 m to the surface. Typically a day and a night tow were taken at each of the six long stations during four seasonally-spaced process cruises (TN043, TN045, TN050, TN054). Occasional additional tows and samples to deeper depths were also done. During the monsoon cruise (TN050), there were often problems with knotted or ripped nets because of the rough seas; data from this cruise should be treated with caution. Tow times and geographic locations can be found in the cruise event log. Cod ends were placed on ice immediately after retrieval. Nets were hosed down with filtered (nominally 2 micron) seawater. For most day tows, the entire sample was preserved in 4% borate-buffered formaldehyde. For most night tows, the samples were split in a NMFS-style (flat-bottomed) plankton splitter. Half the sample was preserved as above for displacement volumes, wet weights, and distributions; one quarter (or the entire remaining half for very small samples and for surface samples) was set aside in a refrigerator for dry weight and CHN subsampling several hours later; and one quarter (when available) was preserved in paraformaldehyde for electron microscopy of zooplankton gut contents. For dry weights, the sample was size-fractionated through a sieve series (2000, 1000, 505, 202 micron) and each size fraction was resuspended in a known volume of filtered seawater. Aliquots were taken with a Stempel piston pipette, filtered onto combusted pre-weighed GF/D 25 mm glass fiber filters, rinsed with distilled water, loosely wrapped in aluminum foil, placed in an oven (60 degrees C) for several days, and stored in a desiccator for shipment home. Two replicates were done for each size fraction. Four blanks (aliquots of filtered seawater treated as above) were done for each tow. In the lab, filters were dried in an oven (60 degrees C) for 8 - 10 hr, weighed for dry weight on a Cahn microbalance, ashed in a muffle furnace (500 degrees C) for 4 hr, and weighed for ash. For dry weights, the pre-cruise filter weight and the mean dry weight blank for the cruise (a single value for each cruise) were subtracted from the measured post-cruise dry weights. The ash-free dry weight was calculated as the post-cruise dry weight minus the post-cruise ash weight and the cruise mean ash weight blank. For carbon values, a conversion factor from the literature was used [log (dry weight) = 0.499 + 0.991 (log carbon)] (Wiebe et al. 1975, Wiebe 1988). The mean of the 2 replicate filters from each sample was used. In cases where no measurements were made and in cases where the weight of a filter with sample was less than the blank and pre-cruise filter weight, the data were recorded as ND (no data). Carbon values from CHN measurements were also obtained by Roman from some of these same samples in the upper 200 m on cruises TN043, TN045, and TN050. Displacement volumes and wet weights were measured several months after the cruise on preserved samples. Either the entire sample or a half split was used. Large singular organisms (large fish or jellyfish) were removed beforehand. Displacement volumes were done with no size fractionation. Wet weights were done on the large (> 2 mm) and small (< 2 mm) size fractions after separation by sieving. Bongo tows were taken at many of the hydro and intermediate stations, as well as the long stations. Wishner's bongo frame (used on TN043, TN045, and the beginning of TN050) had mouth openings of 61 cm, used one net with 153 micron mesh and one with 335 micron mesh, and had a General Oceanics counter flowmeter in each mouth opening. A Wildlife Systems electronic time-depth recorder was attached to the frame. Oblique tows from the starboard side were done to about 200 m depth at about 1.5 - 2 kts. Wire was payed out at 30 m/min and hauled in at 20 m/min. Half of each sample was preserved for displacement volume and distributional measurements, while the other half was used for dry weight, ash-free dry weight, and carbon analyses (and CHN analyses by Roman on the 153 micron mesh net sample) as described above. In the Zooplankton Biomass--MOCNESS and Bongo files, data are presented as mmoles of carbon for each size fraction and for the sum of all size fractions, totaled within the indicated depth range (per m2). For some MOCNESS tows, depth intervals with missing data were represented as the mean of the biomass in the samples immediately above and below in order to calculate a value for the total depth range. References Wiebe, P.H. 1988. Functional regression equations for zooplankton displacement volume, wet weight, dry weight, and carbon: a correction. Fish. Bull. U.S. 86:833-835. Wiebe, P.H., S.H. Boyd, and J.L. Cox. 1975. Relationships between zooplankton displacement volume, wet weight, dry weight, and carbon. Fish. Bull. U.S. 73:777-786. Wiebe, P.H., K.H. Burt, S.H. Boyd, and A.W. Morton. 1976. A multiple opening-closing net and environmental sensing system for sampling zooplankton. J. Mar. Res. 34:312-326. Wiebe, P.H., A.W. Morton, A.M. Bradley, J.E. Craddock, T.J. Cowles, V.A. Barber, R.H. Backus, and G.R. Flierl. 1985. New developments in the MOCNESS, an apparatus for sampling zooplankton and micronekton. Mar. Biol. 87:313-323. Parameters Descriptions Units --------------------------------------------------------------------------- rec header record always 1 ship NODC ship code cruise originator's cruise id lat latitude reported as decimal degrees north (- designates south) long longitude reported as decimal degrees east (- designates west) date/time date/time reported as YYYYMMDDHHmm sta station number rec data record always 3 net Bongo net: 153 or 335 micron mesh zp_C_1 zooplankton carbon, 200-500 micron mmol C/m2 zp_C_2 zooplankton carbon, 500-1000 micron mmol C/m2 zp_C_3 zooplankton carbon, 1000-2000 micron mmol C/m2 zp_C_4 zooplankton carbon, gt 2000 micron mmol C/m2 zp_C_T zooplankton carbon, total size fractions mmol C/m2 --------------------------------------------------------------------------- rec ship cruise lat long date/time sta 1 3250 TTN045 21.1779 63.5320 199503161117 4 rec net zp_C_1 zp_C_2 zp_C_3 zp_C_4 zp_C_T 3 335 3.8 11.1 29.3 26.2 70.4 3 153 15.4 28.9 27.6 34.7 106.6 rec ship cruise lat long date/time sta 1 3250 TTN045 19.8881 65.8861 199503171225 6 rec net zp_C_1 zp_C_2 zp_C_3 zp_C_4 zp_C_T 3 335 2.1 6.7 30 54.6 93.4 3 153 11.9 35.1 53.1 52.2 152.3 rec ship cruise lat long date/time sta 1 3250 TTN045 19.0801 67.1065 199503191506 7 rec net zp_C_1 zp_C_2 zp_C_3 zp_C_4 zp_C_T 3 335 2.3 10.1 78.5 77.1 168 3 153 13.2 30.3 73.1 107.7 224.3 rec ship cruise lat long date/time sta 1 3250 TTN045 17.3234 67.9138 199503201203 9 rec net zp_C_1 zp_C_2 zp_C_3 zp_C_4 zp_C_T 3 335 1.8 5.9 27.5 58.3 93.5 3 153 9.9 12.4 44.7 31 98 rec ship cruise lat long date/time sta 1 3250 TTN045 15.3900 68.7305 199503211343 11 rec net zp_C_1 zp_C_2 zp_C_3 zp_C_4 zp_C_T 3 335 1.4 2.8 19.3 24.3 47.8 3 153 10.7 7.4 32.9 37.8 88.8 rec ship cruise lat long date/time sta 1 3250 TTN045 12.0623 65.0053 199503251058 15 rec net zp_C_1 zp_C_2 zp_C_3 zp_C_4 zp_C_T 3 335 0.8 3 4 7.7 15.5 3 153 5 3.6 10.2 23.5 42.3 rec ship cruise lat long date/time sta 1 3250 TTN045 15.2257 63.4615 199503291023 19 rec net zp_C_1 zp_C_2 zp_C_3 zp_C_4 zp_C_T 3 335 2.1 4.8 25.6 18.9 51.4 3 153 16.1 15.1 34 31.8 97 rec ship cruise lat long date/time sta 1 3250 TTN045 17.7067 58.8083 199504050714 27 rec net zp_C_1 zp_C_2 zp_C_3 zp_C_4 zp_C_T 3 335 1.2 2.7 27.7 32.9 64.5 3 153 4 5.2 37.7 36.1 83