Southern Ocean JGOFS Taxonomic Marker Pigments Data Set (sopig0198.txt) cruise - JGOFS/AESOPS Survey II (KIWI 8) Cruise Dates - 01/21/1998 to 01/25/1998 Platform - Roger A. Revelle Project - Southern Ocean JGOFS/AESOPS Institute - Oregon State University/ORSOO Group =============================================================================================================== 1) Introduction and Methods --------------------------------------------------------------------------------------------------------------- To increase the understanding of biological processes in the Southern Ocean, the concentrations of numerous taxonomic marker pigments were measured during the U.S. Joint Global Ocean Flux Study (JGOFS) Antarctic Environment and Southern Ocean Process Study (AESOPS) Survey II cruise in early 1998. Water samples were collected from the sea surface over a 3o x 2o area near 170oW, 60oS. Pigment concentrations were then measured for each sample using high-pressure liquid chromotography (HPLC). This site presents the resulting pigment maps for this region. The pigment data may be downloaded for use in scientific research after obtaining permission. This study is part of an ongoing research effort in the Southern Ocean by the Oregon State University (O.S.U.) Remote Sensing Ocean Optics (ORSOO) group. Sample collection Pigment samples were collected on the R/V Roger A. Revelle, Survey II, cruise. The sample collection occurred during the same period as a SeaSoar survey. Water was collected from the ship’s surface seawater intake system. Duplicate 2L aliquots were filtered onto GF/F Whatman filters. Filters were frozen and stored in a -80°C freezer until analyzed on shore. Sample analysis Samples were extracted in 3mL of 100% acetone with the addition of 0.1 mL of internal standard canthaxanthin. Samples were sonicated for 90 seconds and extracted for 12 hours in a -20°C freezer. They were then pre-concentrated under constant flow of nitrogen for 10 minutes. Phytoplankton pigment concentrations were determined using a High Pressure Liquid Chromatography (HPLC) system composed of a Perkin-Elmer pump, an Ultrasphere analytical column (150 mm x 4.6 mm), and a SpectraSystem UV2000 absorption detector (dual wavelength, at 436 & 665 nm). The mobile solvent system method was adapted from Wright and Jeffrey (Wright, S. W., and S. W. Jeffrey, "High-resolution HPLC system for chlorophylls and carotenoids of marine phytoplankton", in Phytoplankton Pigments in Oceanography: Guidelines to Modern Methods, eds. S. W. Jeffrey, R. F. Mantoura, and S. W. Wright, Unesco Pub., Paris, pp. 327-341, 1997). The instrument was calibrated using commercial pigment standards (Sigma Chemicals and VKI, Denmark). The pigments studied include: * chlorophyll-a * fucoxanthin * 19'-hexanoyloxyfucoxanthin * chlorophyll-c1 and -c2 * chlorophyll-c3 * 19'-butanoyloxyfucoxanthin * chlorophyll-b * diadinoxanthin =============================================================================================================== 2) Data Format --------------------------------------------------------------------------------------------------------------- The HPLC pigment data (5 kb) are in the following (ASCII) format: rows 1-4: header column 1: sample number column 2: day of year (local time) column 3: local date (MM/DD/YY) column 4: local time (HH:MM) column 5: longitude (oW) column 6: latitude (oS) column 7: chlorophyll-a concentration (ug/l) column 8: fucoxanthin concentration (ug/l) column 9: 19'-hexanoyloxyfucoxanthin concentration (ug/l) column 10: chlorophyll-c1 and -c2 concentration (ug/l)*** column 11: chlorophyll-c3 concentration (ug/l)*** column 12: 19'-butanoyloxyfucoxanthin concentration (ug/l) column 13: chlorophyll-b concentration (ug/l)*** column 14: diadinoxanthin concentration (ug/l)*** ***Note: The chlorophyll-c1and -c2, chlorophyll-c3, chlorophyll-b, and diadinoxanthin concentrations may be overestimated. Re-calculation of these concentrations is currently in progress. =============================================================================================================== 3) Data File --------------------------------------------------------------------------------------------------------------- SURVEY II HPLC pigment concentrations from SEASOAR grid #1 * JD Date Time Long Lat Chla Fuco Hex chlc1&2 chlc3 But Chl b Diadino sample (local) (local) (local) (W) (S) ug/L ug/L ug/L ug/L ug/L ug/L ug/L ug/L 151 21 1/21/98 6:00 171.9 61.272 0.161 0.107 0.049 0.067 0.033 0.021 0.037 0.027 153 21 1/21/98 8:20 171.901 61.017 0.104 0.041 0.029 0.033 0.013 0.01 0.026 0.014 155 21 1/21/98 10:05 171.9 60.823 0.089 0.038 0.028 0.028 0.013 0.011 0.018 0.01 157 21 1/21/98 12:00 171.898 60.612 0.19 0.094 0.039 0.055 0.019 0.015 0.043 0.02 159 21 1/21/98 13:53 171.898 60.402 0.15 0.056 0.058 0.047 0.02 0.019 0.036 0.019 161 21 1/21/98 16:04 171.899 60.167 0.182 0.06 0.086 0.057 0.027 0.021 0.064 0.017 163 21 1/21/98 17:58 171.811 59.999 0.132 0.032 0.071 0.042 0.015 0.015 0.045 0.016 165 21 1/21/98 19:59 171.5 60.065 0.148 0.036 0.081 0.047 0.021 0.018 0.052 0.018 167 21 1/21/98 21:57 171.501 60.272 0.125 0.031 0.073 0.036 0.019 0.017 0.042 0.021 169 22 1/22/98 0:00 171.499 60.497 0.079 0.029 0.026 0.023 0.01 0.012 0.022 0.013 171 22 1/22/98 1:58 171.5 60.74 0.062 0.026 0.019 0.019 0.008 0.01 0.015 0.009 173 22 1/22/98 4:00 171.499 61.005 0.076 0.031 0.024 0.022 0.011 0.01 0.015 0.01 175 22 1/22/98 5:58 171.5 61.229 0.126 0.064 0.035 0.043 0.018 0.015 0.022 0.019 177 22 1/22/98 7:58 171.5 61.469 0.127 0.095 0.035 0.056 0.027 0.017 0.027 0.027 179 22 1/22/98 9:58 171.09 61.504 0.169 0.126 0.022 0.069 0.027 0.011 0.054 0.02 181 22 1/22/98 11:59 171.086 61.281 0.275 0.152 0.049 0.098 0.035 0.022 0.056 0.037 183 22 1/22/98 13:56 171.099 61.059 0.145 0.074 0.031 0.049 0.024 0.015 0.043 0.017 185 22 1/22/98 16:06 171.099 60.811 0.09 0.039 0.031 0.028 0.012 0.012 0.021 0.015 187 22 1/22/98 17:58 171.101 60.588 0.111 0.047 0.042 0.039 0.018 0.013 0.029 0.016 189 22 1/22/98 19:56 171.099 60.338 0.086 0.03 0.035 0.027 0.012 0.01 0.021 0.013 191 22 1/22/98 21:58 171.1 60.075 0.17 0.058 0.071 0.04 0.011 0.022 0.048 0.02 193 22 1/22/98 23:55 170.909 59.916 0.079 0.015 0.033 0.022 0.011 0.009 0.02 0.006 195 23 1/23/98 2:00 170.599 59.998 0.086 0.025 0.041 0.025 0.013 0.011 0.023 0.012 197 23 1/23/98 3:55 170.599 60.212 0.066 0.022 0.036 0.023 0.009 0.009 0.016 0.012 199 23 1/23/98 6:00 170.599 60.449 0.062 0.018 0.021 0.018 0.008 0.007 0.015 0.008 201 23 1/23/98 8:00 170.6 60.668 0.079 0.024 0.025 0.019 0.007 0.008 0.016 0.012 203 23 1/23/98 10:00 170.6 60.893 0.112 0.055 0.036 0.038 0.015 0.012 0.022 0.02 205 23 1/23/98 11:54 170.604 61.119 0.151 0.081 0.033 0.043 0.02 0.014 0.045 0.018 209 23 1/23/98 16:00 170.6 61.617 0.107 0.072 0.02 0.037 0.013 0.01 0.041 0.014 211 23 1/23/98 18:00 170.201 61.605 0.144 0.09 0.02 0.051 0.017 0.01 0.037 0.017 213 23 1/23/98 20:00 170.2 61.369 0.183 0.113 0.024 0.072 0.025 0.01 0.045 0.019 215 23 1/23/98 21:56 170.2 61.128 0.185 0.113 0.042 0.067 0.023 0.02 0.046 0.031 217 23 1/23/98 0:00 170.2 60.872 0.115 0.057 0.034 0.034 0.013 0.011 0.025 0.018 221 24 1/24/98 4:00 170.2 60.405 0.066 0.02 0.025 0.018 0.008 0.007 0.01 0.009 223 24 1/24/98 6:10 170.2 60.159 0.092 0.026 0.043 0.028 0.014 0.011 0.024 0.011 225 24 1/24/98 8:05 170.2 59.932 0.074 0.016 0.033 0.022 0.011 0.008 0.02 0.008 227 24 1/24/98 10:00 169.798 59.955 0.06 0.012 0.023 0.017 0.008 0.006 0.015 0.007 229 24 1/24/98 11:58 169.8 60.182 0.054 0.015 0.023 0.013 0.005 0.006 0.02 0.005 231 24 1/24/98 14:03 169.779 60.368 0.107 0.043 0.026 0.029 0.009 0.016 0.028 0.01 233 24 1/24/98 16:07 169.796 60.478 0.064 0.021 0.02 0.019 0.008 0.011 0.016 0.009 235 24 1/24/98 18:00 169.8 60.713 0.109 0.051 0.036 0.036 0.017 0.014 0.027 0.018 237 24 1/24/98 21:00 169.799 61.099 0.141 0.089 0.035 0.051 0.019 0.019 0.029 0.029 239 24 1/24/98 23:54 169.799 61.464 0.111 0.107 0.025 0.062 0.024 0.015 0.031 0.03 241 25 1/25/98 3:03 169.4 61.367 0.071 0.073 0.019 0.044 0.019 0.012 0.025 0.018 245 25 1/25/98 9:00 169.401 60.648 0.035 0.017 0.014 0.014 0.007 0.006 0.008 0.008 247 25 1/25/98 12:00 169.4 60.284 0.042 0.017 0.018 0.015 0.008 0.005 0.017 0.006 249 25 1/25/98 15:08 169.4 59.892 0.079 0.025 0.025 0.019 0.006 0.011 0.025 0